Monitoring the Cycle
Occasionally, it is necessary to intervene in the natural mating process when natural breeding is unsuccessful. If it appears that the female has a problem conceiving, or if she refuses the male when all else appears normal, several avenues are available.
A series of hormonal blood tests can be done to determine when she is truly ready to ovulate and thus should accept a male. As the female nears ovulation, certain hormones will rise, indicating the timing for insemination.
Examining vaginal cytology is also a helpful tool to map out a cycle. The local cell morphology will mature as the dog nears ovulation.